BADEJO Adebanjo Ayobamidele (Publications)
Publisher PhD., DR. BADEJO, A.A.
Publication Type Disertations
Publication Title Engineering Increased Vitamin C Levels In Plants With The Genes Of Acerola(Malpighiaglabra).
Publication Authors DR. BADEJO, A.A.
Year Published 2009
Abstract

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Publisher Applied Tropical Agriculture 7: 6-11, Enujiugha, V.N. And Badejo, A.A.
Publication Type Journal
Publication Title Cultural Alteration For The Improvement Of Bacillus Subtilis In The Fermentation Of African Oil Bean (Pentaclethramacrophylla) Seed.
Publication Authors Enujiugha, V.N. and Badejo, A.A.
Year Published 2002
Abstract

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Publisher Journal Of Food Agriculture Environment. 1: 72-75, Enujiugha, V.N., Badejo, A.A.,Iyiola, S.O. And Oluwamukomi, M.O.
Publication Type Journal
Publication Title Effect Of Germination On The Nutritional And Functional Properties Of African Oil Bean (Pentaclethramacrophylla) Seed Flour.
Publication Authors Enujiugha, V.N., Badejo, A.A.,Iyiola, S.O. and Oluwamukomi, M.O.
Year Published 2003
Abstract

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Publisher Oxford University Press
Publication Type Journal
Publication Title Analysis Of GDP-d-Mannose Pyrophosphorylase Gene Promoter From Acerola (Malpighia Glabra) And Increase In Ascorbate Content Of Transgenic Tobacco Expressing The Acerola Gene
Publication Authors Badejo, A.A., Tanaka, N. and Esaka, M.
Year Published 2008
Abstract

GDP-d-mannose pyrophosphorylase (GMP) is an important enzyme in the Smirnoff–Wheelers pathway for the biosynthesis of ascorbic acid (AsA) in plants. We have reported recently that the expression of the acerola (Malpighia glabra) GMP gene, designated MgGMP, correlates with the AsA content of the plant. The acerola plant has very high levels of AsA relative to better studied model plants such as Arabidopsis. Here we found that the GMP mRNA levels in acerola are higher than those from Arabidopsis and tomato. Also, the transient expression of the uidA reporter gene in the protoplasts of Nicotiana tabacum cultures showed the MgGMP gene promoter to have higher activity than the cauliflower mosaic virus 35S and Arabidopsis GMP promoters. The AsA content of transgenic tobacco plants expressing the MgGMP gene including its promoter was about 2-fold higher than that of the wild type.


Publisher Elsevier
Publication Type Journal
Publication Title Cloning And Expression Of GDP-d-mannose Pyrophosphorylase Gene And Ascorbic Acid Content Of Acerola (Malpighia Glabra L.) Fruit At Ripening Stages
Publication Authors Badejo, A.A., Jeong, S.T., Goto-Yamamoto, N. and Esaka, M
Year Published 2007
Abstract

Acerola (Malpighia glabra L.) is one of the richest natural sources of l-ascorbic acid (AsA; vitamin C). GDP-d-mannose pyrophosphorylase (GMP; EC 2.7.7.13) was found to play a major role in the proposed AsA biosynthetic pathway in plants, considering that Arabidopsis vtc1-1 mutant with point mutation in this gene has a highly reduced AsA content. GMP cDNA was isolated from acerola fruits, designated MgGMP, using rapid amplification of cDNA ends (RACE), and its expression was monitored during fruit ripening. The full-length cDNA was found to have an ORF of 1083 bp encoding a polypeptide of 361 amino acids. In silico analysis of the predicted amino acid sequence showed a pI of 6.45 and molecular mass of 39.7 kD. MgGMP showed over 80% amino acid sequence identity with other plant GMP homologues. The phylogenetic tree shows the close relation of MgGMP to the GMP of other plants as against those from parasite, yeasts and mammals. Southern analysis indicated that M. glabra contains not less than two copies of GMP genes. Northern blot analysis showed the transcript abundance of MgGMP in all the organs of acerola examined, with the fruit having the highest expression. The relative transcript abundance of MgGMP mRNA levels in the fruits changes as the ripening process progresses, with the unripe green fruits having the highest relative mRNA level, and the lowest was found in the fruits at advanced ripening stage. A strong correlation was also observed between the relative MgGMP mRNA levels and the AsA contents of acerola during fruit ripening.


Publisher Elsevier
Publication Type Journal
Publication Title Gene Expression Of Ascorbic Acid Biosynthesis Related Enzymes Of The Smirnoff-Wheeler Pathway In Acerola (Malpighia Glabra)
Publication Authors Badejo, A.A., Fujikawa, Y. and Esaka, M.
Year Published 2009
Abstract

The Smirnoff-Wheeler (SW) pathway has been proven to be the only significant source of l-ascorbic acid (AsA; vitamin C) in the seedlings of the model plant Arabidopsis thaliana. It is yet uncertain whether the same pathway holds for all other plants and their various organs as AsA may also be synthesized through alternative pathways. In this study, we have cloned some of the genes involved in the SW-pathway from acerola (Malpighia glabra), a plant containing enormous amount of AsA, and examined the expression patterns of these genes in the plant. The AsA contents of acerola leaves were about 8-fold more than that of Arabidopsis with 5-700-fold higher mRNA abundance in AsA-biosynthesizing genes. The unripe fruits have the highest AsA content but the accumulation was substantially repressed as the fruit transitions to maturation. The mRNAs encoding these genes showed correlation in their expression with the AsA contents of the fruits. Although very little AsA was recorded in the seeds the mRNAs encoding all the genes, with the exception of the mitochondrially located l-galactono-1,4-lactone dehydrogenase, were clearly detected in the seeds of the unripe fruits. In young leaves of acerola, the expression of most genes were repressed by the dark and induced by light. However, the expression of GDP-d-mannose pyrophosphorylase similar to that encoded by A. thaliana VTC1 was induced in the dark. The expressions of all the genes surged after 24 h following wounding stress on the young leaves. These findings will advance the investigation into the molecular factors regulating the biosynthesis of abundant AsA in acerola.


Publisher Oxford Press
Publication Type Journal
Publication Title Translocation And The Alternative D-galacturonate Pathway Contribute To Increasing The Ascorbate Level In Ripening Tomato Fruits Together With The D-mannose/L-galactose Pathway
Publication Authors Badejo AA, Wada K, Gao Y, Maruta T, Sawa Y, Shigeoka S, Ishikawa T.
Year Published 2012
Abstract

The D-mannose/L-galactose pathway for the biosynthesis of vitamin C (L-ascorbic acid; AsA) has greatly improved
the understanding of this indispensable compound in plants, where it plays multifunctional roles. However, it is yet
to be proven whether the same pathway holds for all the different organs of plants, especially the fruit-bearing
plants, at different stages of development. Micro-Tom was used here to elucidate the mechanisms of AsA
accumulation and regulation in tomato fruits. The mRNA expression of the genes in the D-mannose/L-galactose
pathway were inversely correlated with increasing AsA content of Micro-Tom fruits during ripening. Feeding L-
[6-14C]AsA to Micro-Tom plants revealed that the bulk of the label from AsA accumulated in the source leaf was
transported to the immature green fruits, and the rate of translocation decreased as ripening progressed. LGalactose
feeding, but neither D-galacturonate nor L-gulono-1,4-lactone, enhanced the content of AsA in immature
green fruit. On the other hand, L-galactose and D-galacturonate, but not L-gulono-1,4-lactone, resulted in an increase
in the AsA content of red ripened fruits. Crude extract prepared from insoluble fractions of green and red fruits
showed D-galacturonate reductase- and aldonolactonase-specific activities, the antepenultimate and penultimate
enzymes, respectively, in the D-galacturonate pathway, in both fruits. Taken together, the present findings
demonstrated that tomato fruits could switch between different sources for AsA supply depending on their ripening
stages. The translocation from source leaves and biosynthesis via the D-mannose/L-galactose pathway are dominant
sources in immature fruits, while the alternative D-galacturonate pathway contributes to AsA accumulation in
ripened Micro-Tom fruits.


Publisher ND., DR. BADEJO, A.A.
Publication Type Disertations
Publication Title Physical And Chemical Analysis Of Cocoa Based Drink.
Publication Authors DR. BADEJO, A.A.
Year Published 1994
Abstract

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Publisher B.Tech., DR. BADEJO, A.A.
Publication Type Disertations
Publication Title Cultural Alteration For The Improvement Of Bacillus Subtilis In The Fermentation Of African Oil Bean (Pentaclethramacrophylla) Seed.
Publication Authors DR. BADEJO, A.A.
Year Published 2000
Abstract

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Publisher M.Sc, DR. BADEJO, A.A.
Publication Type Disertations
Publication Title Cloning And Expression Of Ascorbic Acid Biosynthesis Related Enzymes From Acerola (Malpighiaglabra).
Publication Authors DR. BADEJO, A.A.
Year Published 2006
Abstract

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Publisher Pp405-428. Springer, Netherland, Badejo, A.A. And Esaka, M.
Publication Type Journal
Publication Title Identification Of Potential Gene Targets For The Improvement Of Ascorbate Contents Of Genetically Modified Plants. In N.A. Anjum (Eds.), Ascorbate-Glutathione Pathway And Stress Tolerance In Plants.
Publication Authors Badejo, A.A. and Esaka, M.
Year Published 2010
Abstract

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Publisher Plant Physiology And Biochemistry 45: 665-672, Badejo, A.A.,Jeong S.T., Goto-Yamamoto N. And Esaka M.
Publication Type Journal
Publication Title Cloning And Expression Of GDP-D-mannose Pyrophosphorylase Gene And Ascorbic Acid Content Of Acerola (Malpighiaglabra L.) Fruit At Ripening Stages.
Publication Authors Badejo, A.A.,Jeong S.T., Goto-Yamamoto N. and Esaka M.
Year Published 2007
Abstract

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Publisher ActaHorticulturae (ISHS) 763: 91-98, Badejo, A.A.,Esaka, M., Jeong, S.T. And Goto-Yamamoto, N.
Publication Type Journal
Publication Title Molecular Cloning And Expression Of GDP-D-mannose-3,5- Epimerase During Fruit Ripening In Acerola.
Publication Authors Badejo, A.A.,Esaka, M., Jeong, S.T. and Goto-Yamamoto, N.
Year Published 2007
Abstract

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Publisher Plant And Cell Physiology 49: 126-132, Badejo, A.A., Tanaka, N. And Esaka, M.
Publication Type Journal
Publication Title Analysis Of GDP-D-mannose Pyrophosphorylase Gene Promoter From Acerola (Malpighiaglabra) And Increase In Ascorbate Content Of Transgenic Tobacco Expressing Acerola Gene.
Publication Authors Badejo, A.A., Tanaka, N. and Esaka, M.
Year Published 2008
Abstract

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Publisher Journal Of Plant Physiology 166: 652-660, Badejo, A.A.,Fujikawa, Y. And Esaka, M.
Publication Type Journal
Publication Title Gene Expression Of Ascorbic Acid Biosynthesis Related Enzymes Of Smirnoff-Wheeler Pathway In Acerola (Malpighiaglabra).
Publication Authors Badejo, A.A.,Fujikawa, Y. and Esaka, M.
Year Published 2009
Abstract

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Publisher Plant And Cell Physiology 50: 423-428, Badejo, A.A.,Eltelib, H.A., Fukunaga, K., Fujikawa, Y. And Esaka, M.
Publication Type Journal
Publication Title Increase In Ascorbate Content Of Transgenic Tobacco Plants Overexpressing Acerola (Malpighiaglabra) Phosphomannomutase Gene.
Publication Authors Badejo, A.A.,Eltelib, H.A., Fukunaga, K., Fujikawa, Y. and Esaka, M.
Year Published 2009
Abstract

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Publisher Journal Of Graduate School Of Biosphere Science, Hiroshima University 48: 123-125, Badejo, A.A.
Publication Type Journal
Publication Title Engineering Increased Vitamin C Levels In Plants With The Genes Of Acerola (Malpighiaglabra).
Publication Authors Badejo, A.A.
Year Published 2009
Abstract

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Publisher Horticulture, Environment, And Biotechnology 50 (4): 1-5, Badejo, A.A.,EltelibH.A., Fujikawa Y And Esaka M.
Publication Type Journal
Publication Title Genetic Manipulation For Enhancing Vitamin C Content In Tobacco Expressing Acerola (Malpighiaglabra) GDP-L-galactose Phosphorylase Gene.
Publication Authors Badejo, A.A.,EltelibH.A., Fujikawa Y and Esaka M.
Year Published 2009
Abstract

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Publisher Journal Of Plant Physiology 168 (6): 619-627, Eltelib, H.A., Badejo, A.A.,Fujikawa, Y And Esaka, M.
Publication Type Journal
Publication Title Gene Expression Of Monodehydroascorbate Reductase And Dehydroascorbatereductase During Fruit Ripening And In Response To Environmental Stresses In Acerola (Malpighiaglabra).
Publication Authors Eltelib, H.A., Badejo, A.A.,Fujikawa, Y and Esaka, M.
Year Published 2010
Abstract

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Publisher Journal Of Experimental Botany 62 (10): 3647-3657, Gao, Y., Nishikawa, H., Badejo, A.A., Shibata. H., Sawa, Y., Nakagawa, T., Maruta, T., Shigeru, S., Smirnoff, N. And Ishikawa, T.
Publication Type Journal
Publication Title Expression Of Aspartyl Protease And C3HC4-type RING Zinc Finger Genes Are Responsive To Ascorbic Acid In Arabidopsis Thaliana.
Publication Authors Gao, Y., Nishikawa, H., Badejo, A.A., Shibata. H., Sawa, Y., Nakagawa, T., Maruta, T., Shigeru, S., Smirnoff, N. and Ishikawa, T.
Year Published 2011
Abstract

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Publisher Bioscience, Biotechnology, And Biochemistry 75 (9): 1783-1788, Gao, Y., Badejo, A.A., Shibata, H., Sawa, Y., Maruta, T., Shigeru, S., Page, M., Smirnoff, N. And Ishikawa, T.
Publication Type Journal
Publication Title Expression Analysis Of The VTC2 And VTC5 Genes Encoding GDP-L-galactose Phosphorylase, An Enzyme Involved In Ascorbate Biosynthesis, In Arabidopsis.
Publication Authors Gao, Y., Badejo, A.A., Shibata, H., Sawa, Y., Maruta, T., Shigeru, S., Page, M., Smirnoff, N. and Ishikawa, T.
Year Published 2011
Abstract

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Publisher Journal Of Experimental Botany63 (1) 229-239, Badejo, A.A., Wada, K., Gao, Y., Maruta, T., Sawa, Y., Shigeru, S And Ishikawa, T.
Publication Type Journal
Publication Title Translocation And The Alternative D-galacturonate Pathway Contribute To Increasing The Ascorbate Level In Ripening Tomato Fruits Together With The D-mannose/L-galactose Pathway.
Publication Authors Badejo, A.A., Wada, K., Gao, Y., Maruta, T., Sawa, Y., Shigeru, S and Ishikawa, T.
Year Published 2012
Abstract

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Publisher Plant And Cell Physiology53(3): 592-601, Gao, Y., Badejo, A.A.,Sawa, Y. And Ishikawa,T.
Publication Type Journal
Publication Title Analysis Of Two L-galactono-1,4-lactone Responsive Genes With Complementary Expression During The Development Of Arabidopsis Thaliana.
Publication Authors Gao, Y., Badejo, A.A.,Sawa, Y. and Ishikawa,T.
Year Published 2012
Abstract

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Publisher Food 6 (1): 38-43, Fagbemi, T.N., Adeoya, A.S. And Badejo, A.A.
Publication Type Journal
Publication Title Effect Of Sulphiting On The Physical And Functional Properties Of Acetylated Cassava (Manihotesculenta) Starch.
Publication Authors Fagbemi, T.N., Adeoya, A.S. and Badejo, A.A.
Year Published 2012
Abstract

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Publisher World Academy Of Science, Engineering And Technology 69: 2082-2086, Awe, F., Fagbemi, T.N., Ajibola, C.F. And Badejo, A.A.
Publication Type Journal
Publication Title The Antioxidant Capacity Of Beverage Blends Made From Cocoa, Zobo And Ginger.
Publication Authors Awe, F., Fagbemi, T.N., Ajibola, C.F. and Badejo, A.A.
Year Published 2012
Abstract

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Publisher Food Research International 52: 490-495, Awe, F., Fagbemi, T.N., Ifesan, B.O.T. And Badejo, A.A.
Publication Type Journal
Publication Title Antioxidant Properties Of Cold And Hot Water Extract Of Cocoa, Hibiscus Flower Extract And Ginger Beverage Blends.
Publication Authors Awe, F., Fagbemi, T.N., Ifesan, B.O.T. and Badejo, A.A.
Year Published 2013
Abstract

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Publisher Raw Materials And Research Development Council, Ondo State, Fagbemi, T.N. And Badejo, A.A.
Publication Type Technical Papers
Publication Title Strategies For Sustainable Processing And Preservation Of Tropical Fruits In Ondo State.
Publication Authors Fagbemi, T.N. and Badejo, A.A.
Year Published 2012
Abstract

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Publisher 23rd Annual NIFST Conference, Enujiugha, V.N. And Badejo, A.A.
Publication Type Conference
Publication Title Effect Of Changes In The Culture Conditions Of Bacillus Subtilis For African Oil Bean (Pentaclethramacrophylla) Fermentation.
Publication Authors Enujiugha, V.N. and Badejo, A.A.
Year Published 1999
Abstract

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Publisher The First World Congress Of Industrial Biotechnology, Hangzhou, Peoples Republic Of China, Badejo, A.A. And Esaka, M.
Publication Type Conference
Publication Title The Biosynthetic Pathway Of Vitamin C In Acerola (Malpighiaglabra).
Publication Authors Badejo, A.A. and Esaka, M.
Year Published 2008
Abstract

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Publisher Japan Society For Bioscience, Biotechnology And Agronomy Congress, Okinawa, Japan, Eltelib, H.A., Badejo, A.A.,Fujikawa, Y. And Esaka, M.
Publication Type Conference
Publication Title Gene Expression Of Vitamin C Biosynthesis And Metabolism Related Enzymes In Exotic Fruit-acerola.
Publication Authors Eltelib, H.A., Badejo, A.A.,Fujikawa, Y. and Esaka, M.
Year Published 2009
Abstract

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Publisher 21st International Conference On Arabidopsis Research, Yokohama, Japan, Badejo, A.A.and Esaka, M.
Publication Type Conference
Publication Title Higher Expression Of Vitamin C Biosynthesis Genes Leads To Higher Vitamin C Contents In Acerola (Malpighiaglabra) Compared To Arabidopsis Thaliana.
Publication Authors Badejo, A.A.and Esaka, M.
Year Published 2010
Abstract

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Publisher 33rd Annual Meeting Of The Molecular Biology Society Of Japan. Port-Island Kobe, Japan, Wada, K., Badejo, A.A.,Sawa, Y., Shibata, H., Maruta, T., Shigeoka, S. And Ishikawa T.
Publication Type Conference
Publication Title The Expression Of Ascorbic Acid Biosynthesis Genes During Ripening In Tomato Fruits.
Publication Authors Wada, K., Badejo, A.A.,Sawa, Y., Shibata, H., Maruta, T., Shigeoka, S. and Ishikawa T.
Year Published 2010
Abstract

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Publisher 52nd Annual Meeting Of The Japanese Society Of Plant Physiologists. Tohoku University Sendai, Miyagi Prefecture Japan, Gao, Y., Masuzawa, T., Badejo, A.A., Shibata, H., Sawa, Y., Smirnoff, N., Maruta, T., Shigeoka, S. And Ishikawa, T.
Publication Type Conference
Publication Title Expression Analysis Of VTC2 And VTC5 Genes Related To Ascorbate Biosynthesis In Arabidopsis.
Publication Authors Gao, Y., Masuzawa, T., Badejo, A.A., Shibata, H., Sawa, Y., Smirnoff, N., Maruta, T., Shigeoka, S. and Ishikawa, T.
Year Published 2011
Abstract

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Publisher 52nd Annual Meeting Of The Japanese Society Of Plant Physiologists, Badejo, A.A.,Arita, M., Shibata, H., Sawa, Y., Smirnoff, N., Maruta, T., Shigeoka, S. And Ishikawa, T.
Publication Type Conference
Publication Title Can NDPK Contribute To The Regulation Of Ascorbate Biosynthesis In Arabidopsis?
Publication Authors Badejo, A.A.,Arita, M., Shibata, H., Sawa, Y., Smirnoff, N., Maruta, T., Shigeoka, S. and Ishikawa, T.
Year Published 2011
Abstract

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Publisher Annual Meeting Of The Japan Society For Bioscience, Biotechnology, And Agrochemistry, Wada, K., Badejo, A.A., Sawa, Y., Shibata, H., Maruta T., Shigeoka S. And Ishikawa T.
Publication Type Conference
Publication Title Control Of Ascorbate Accumulation In Tomato (Lycopersicumesculentum) Fruits.
Publication Authors Wada, K., Badejo, A.A., Sawa, Y., Shibata, H., Maruta T., Shigeoka S. and Ishikawa T.
Year Published 2011
Abstract

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Publisher 63rd Annual Meeting Of The Vitamin Society Of Japan, Badejo, A.A., Wada, K., Shibata, H., Sawa, Y., Maruta T., Shigeoka S. And Ishikawa T.
Publication Type Conference
Publication Title Vitamin C Biosynthesis And Translocation In Tomato (Lycopersicumesculentum) Fruits.
Publication Authors Badejo, A.A., Wada, K., Shibata, H., Sawa, Y., Maruta T., Shigeoka S. and Ishikawa T.
Year Published 2011
Abstract

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Publisher 10th International Conference On Reactive Oxygen And Nitrogen Species In Plants, Badejo, A.A., Wada, K., Maruta T., Sawa, Y., Shigeoka S. And Ishikawa T.
Publication Type Conference
Publication Title Regulation Of Ascorbate Pool Size In Tomato (Lycopersicumesculentum) Fruits.
Publication Authors Badejo, A.A., Wada, K., Maruta T., Sawa, Y., Shigeoka S. and Ishikawa T.
Year Published 2011
Abstract

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Publisher 8thSolanaceae And 2ndCucurbitaceae Joint Conference, Badejo, A.A.,Maruta T., Sawa, Y., Shigeoka S. And Ishikawa T.
Publication Type Conference
Publication Title Unfolding The Mechanism Of Vitamin C Accumulation In Ripening Micro-Tom Fruits.
Publication Authors Badejo, A.A.,Maruta T., Sawa, Y., Shigeoka S. and Ishikawa T.
Year Published 2011
Abstract

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Publisher 50thCanadian Institute Of Food Science And Technology Conference, Awe,F.B., Fagbemi, T.N., Ifesan, B.O.T. And Badejo, A.A.
Publication Type Conference
Publication Title Antioxidant Properties Of Cold And Hot Water Extract Of ‘Cocoa::Zobo::Ginger’ Beverage Blends.
Publication Authors Awe,F.B., Fagbemi, T.N., Ifesan, B.O.T. and Badejo, A.A.
Year Published 2012
Abstract

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Publisher Half Year Conference Of The Nigerian Institute Of Food Science And Technology, Western Chapter. University Of Ilorin, Ilorin, Nigeria, Awe,F.B., Fagbemi, T.N., Ajibola C.F. And Badejo, A.A.
Publication Type Conference
Publication Title Analysis Of The Antioxidant Potentialsof Beverage Blends Developed From Cocoa, Zobo And Ginger.
Publication Authors Awe,F.B., Fagbemi, T.N., Ajibola C.F. and Badejo, A.A.
Year Published 2012
Abstract

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Publisher World Academy Of Science And Engineering, Awe, F.B., Fagbemi, T.N., Ajibola C.F. And Badejo, A.A.
Publication Type Conference
Publication Title The Antioxidant Capacity Of Beverage Blends Made From Cocoa, Zobo And Ginger.
Publication Authors Awe, F.B., Fagbemi, T.N., Ajibola C.F. and Badejo, A.A.
Year Published 2012
Abstract

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Publisher 16th Triennial Symposium Of The International Society For Tropical Root Crops, Fagbemi, T.N., Adeoya, A.S. AndBadejo, A.A.
Publication Type Conference
Publication Title Effect Of Acetylation On Some Physical And Functional Properties Of Cassava Starch.
Publication Authors Fagbemi, T.N., Adeoya, A.S. andBadejo, A.A.
Year Published 2012
Abstract

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